Speaking to state TV from outside a ruined building, he asserted: “I am in Tripoli and not in Venezuela.”
UK Foreign Minister William Hague had said he had seen information suggesting Col Gaddafi was on his way to Caracas.
Earlier, the new General Committee for Defence said its forces would cleanse Libya of anti-government elements.
A statement described the protesters as “terrorist gangs made up mostly of misguided youths”, who had been exploited and fed “hallucinogenic pills” by people following foreign agendas.
Full Story: BBC News – Libyan leader Muammar Gaddafi appears on state TV